HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43], Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43], Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-33-732
• Hersteller Artikelnummer: 33-732
• Alternativnummer: PRS-33-732-100
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
• Konjugation: Unconjugated

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [CR3/43]
• NCBI: 311531173119
• UniProt: P04440
• Puffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher
• Anwendungsbeschreibung: WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.

Bilder

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10 6 cells (blocked with goat sera), Red=isotype control, Blue= HLA-DP/DQ/DR antibody.

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).