HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-33-732
• Supplier Catalog Number: 33-732
• Alternative Catalog Number: PRS-33-732-100
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
• Conjugation: Unconjugated

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [CR3/43]
• NCBI: 311531173119
• UniProt: P04440
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher
• Application Notes: WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.

Images

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10 6 cells (blocked with goat sera), Red=isotype control, Blue= HLA-DP/DQ/DR antibody.

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).