Caspase-8 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Caspase-8 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-3473
• Hersteller Artikelnummer: 3473
• Alternativnummer: PRS-3473-0.02,PRS-3473-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
• Konjugation: Unconjugated
• Alternative Synonym: Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, CASP-8

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: 55 kDa
• NCBI: 841
• UniProt: Q14790
• Puffer: Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL and immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Bilder

Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 &956,g/mL in (A) the absence and (B) the presence of blocking peptide.

Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 &956,g/ml.

Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 &956,g/mL.

Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 &956,g/mL.

Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 &956,g/ml.Red: Caspase-8 Antibody (3473) Blue: DAPI staining

Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 &956,g/mL.