Caspase-8 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Caspase-8 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3473
• Supplier Catalog Number: 3473
• Alternative Catalog Number: PRS-3473-0.02,PRS-3473-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
• Conjugation: Unconjugated
• Alternative Names: Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, CASP-8

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: 55 kDa
• NCBI: 841
• UniProt: Q14790
• Buffer: Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL and immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 &956,g/mL in (A) the absence and (B) the presence of blocking peptide.

Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 &956,g/ml.

Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 &956,g/mL.

Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 &956,g/mL.

Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 &956,g/ml.Red: Caspase-8 Antibody (3473) Blue: DAPI staining

Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 &956,g/mL.