WNT-3a Antibody (biotin), Biotin, Rabbit, Polyclonal

Produktübersicht

• Artikelname: WNT-3a Antibody (biotin), Biotin, Rabbit, Polyclonal
• Artikelnummer: PRS-38-178
• Hersteller Artikelnummer: 38-178
• Alternativnummer: PRS-38-178-0.05
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.
• Konjugation: Biotin
• Alternative Synonym: Protein Wnt-3a

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 89780
• UniProt: P56704
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: ELISA:Sandwich:To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot:To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.