WNT-3a Antibody (biotin), Biotin, Rabbit, Polyclonal

Catalog Number: PRS-38-178
Article Name: WNT-3a Antibody (biotin), Biotin, Rabbit, Polyclonal
Biozol Catalog Number: PRS-38-178
Supplier Catalog Number: 38-178
Alternative Catalog Number: PRS-38-178-0.05
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.
Conjugation: Biotin
Alternative Names: Protein Wnt-3a
Clonality: Polyclonal
Concentration: batch dependent
NCBI: 89780
UniProt: P56704
Form: Lyophilized
Application Dilute: Centrifuge vial prior to opening.
Application Notes: ELISA:Sandwich:To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot:To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.