NT-3 Antibody (biotin), Biotin, Rabbit, Polyclonal

Artikelnummer: PRS-38-334
Artikelname: NT-3 Antibody (biotin), Biotin, Rabbit, Polyclonal
Artikelnummer: PRS-38-334
Hersteller Artikelnummer: 38-334
Alternativnummer: PRS-38-334-0.025
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography and then biotinylated.
Konjugation: Biotin
Alternative Synonym: Neurotrophin-3, Neurotrophic factor, Nerve Growth Factor-2 (NGF-2), HGNF
Klonalität: Polyclonal
Konzentration: batch dependent
NCBI: 4908
UniProt: P20783
Formulierung: Lyophilized
Application Verdünnung: Centrifuge vial prior to opening.
Anwendungsbeschreibung: Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Human NT-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3. Western Blot To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.