NT-3 Antibody (biotin), Biotin, Rabbit, Polyclonal
Catalog Number:
PRS-38-334
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| Article Name: | NT-3 Antibody (biotin), Biotin, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-38-334 |
| Supplier Catalog Number: | 38-334 |
| Alternative Catalog Number: | PRS-38-334-0.025 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation: | Biotin |
| Alternative Names: | Neurotrophin-3, Neurotrophic factor, Nerve Growth Factor-2 (NGF-2), HGNF |
| Application Dilute: | Centrifuge vial prior to opening. |
| Application Notes: | Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Human NT-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3. Western Blot To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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