Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Humanbeta-NGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Humanbeta-NGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Humanbeta-NGF. Western Blot To detect Humanbeta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Humanbeta-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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