beta-NGF Antibody (biotin), Biotin, Rabbit, Polyclonal

Product Overview

• Article Name: beta-NGF Antibody (biotin), Biotin, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-38-336
• Supplier Catalog Number: 38-336
• Alternative Catalog Number: PRS-38-336-0.025
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Biotin
• Alternative Names: NGF-beta, beta-Nerve Growth Factor

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• NCBI: 4803
• UniProt: P01138
• Form: Lyophilized

Application Notes

• Application Dilute: Centrifuge vial prior to opening.
• Application Notes: Sandwich ELISA: To detect Humanbeta-NGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Humanbeta-NGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Humanbeta-NGF. Western Blot To detect Humanbeta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Humanbeta-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.