H1N1 Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-5245
Artikelname: H1N1 Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-5245
Hersteller Artikelnummer: 5245
Alternativnummer: PRS-5245-0.02,PRS-5245-0.1
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Virus
Immunogen: Neuraminidase antibody was raised against synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein.The antibody recognizes both variants.The immunogen is located within the first 50 amino acids of H1N1 Neuraminidase.
Konjugation: Unconjugated
Alternative Synonym: H1N1 Neuraminidase Antibody:
Klonalität: Polyclonal
Konzentration: 1 mg/mL
UniProt: B1AGS8
Puffer: H1N1 Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 2 µg/mL. Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the seasonal influenza A (H1N1) and the novel swine influenza in ELISA and WB. It will detect 2 ng of free peptide at 1 µg/mL.
Figure 2 ELISA Validation with H1N1 NA Protein Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 &956,g/mL, incubated at 4 &730,C overnight. Detection Antibodies: H1N1 NA antibody, 5245, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.
Figure 1 Western Blot Validation with H1N1 NA Protein Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 5245, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.