H1N1 Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: H1N1 Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-5245
• Supplier Catalog Number: 5245
• Alternative Catalog Number: PRS-5245-0.02,PRS-5245-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Neuraminidase antibody was raised against synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein.The antibody recognizes both variants.The immunogen is located within the first 50 amino acids of H1N1 Neuraminidase.
• Conjugation: Unconjugated
• Alternative Names: H1N1 Neuraminidase Antibody:

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• UniProt: B1AGS8
• Buffer: H1N1 Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 2 µg/mL. Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the seasonal influenza A (H1N1) and the novel swine influenza in ELISA and WB. It will detect 2 ng of free peptide at 1 µg/mL.

Images

Figure 2 ELISA Validation with H1N1 NA Protein Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 &956,g/mL, incubated at 4 &730,C overnight. Detection Antibodies: H1N1 NA antibody, 5245, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.

Figure 1 Western Blot Validation with H1N1 NA Protein Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 5245, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.