HSPA5 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HSPA5 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-57-011
• Hersteller Artikelnummer: 57-011
• Alternativnummer: PRS-57-011-200
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
• Konjugation: Unconjugated
• Alternative Synonym: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: 72 kDa
• NCBI: 3309
• UniProt: P11021
• Puffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Bilder

Antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.

Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Confocal immunofluorescent analysis of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.