HSPA5 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HSPA5 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-57-011
• Supplier Catalog Number: 57-011
• Alternative Catalog Number: PRS-57-011-200
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
• Conjugation: Unconjugated
• Alternative Names: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: 72 kDa
• NCBI: 3309
• UniProt: P11021
• Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Images

Antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.

Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Confocal immunofluorescent analysis of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.