LIF Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: LIF Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-6245
• Hersteller Artikelnummer: 6245
• Alternativnummer: PRS-6245-0.02,PRS-6245-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF.
• Konjugation: Unconjugated
• Alternative Synonym: LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: Predicted: 22kDObserved: 26kD (Post-modifications: N-linked Glycosylations)
• NCBI: 3976
• UniProt: P15018
• Puffer: LIF Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1-4 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Bilder

Figure 1 Western Blot Validation in Mouse 3T3 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of LIF in Mouse 3T3 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with 6245 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation with Recombinant ProteinLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1: 1 &956,g/mL, 2: 2&956,g/mL, 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Mouse Cell Lines and TissueLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Western Blot Validation in Rat LungLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1: 1 &956,g/mL, 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.