LIF Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: LIF Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-6245
• Supplier Catalog Number: 6245
• Alternative Catalog Number: PRS-6245-0.02,PRS-6245-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF.
• Conjugation: Unconjugated
• Alternative Names: LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 22kDObserved: 26kD (Post-modifications: N-linked Glycosylations)
• NCBI: 3976
• UniProt: P15018
• Buffer: LIF Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1-4 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Images

Figure 1 Western Blot Validation in Mouse 3T3 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of LIF in Mouse 3T3 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with 6245 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation with Recombinant ProteinLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1: 1 &956,g/mL, 2: 2&956,g/mL, 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Mouse Cell Lines and TissueLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Western Blot Validation in Rat LungLoading: 15 &956,g of lysates per lane.Antibodies: LIF 6245, (1: 1 &956,g/mL, 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.