FGF-17 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: FGF-17 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-XP-5133
• Hersteller Artikelnummer: XP-5133
• Alternativnummer: PRS-XP-5133-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17).
• Konjugation: Unconjugated
• Alternative Synonym: HH20, FGF-13Fibroblast growth factor 17FGF-17

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 8822
• UniProt: O60258
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/mL), a concentration of 0.02 - 0.035 µg/mL of this antibody is required. ELISA:To detect hFGF-17 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-17 (XP-5133Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot:To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.