FGF-17 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-XP-5133
Article Name: FGF-17 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-XP-5133
Supplier Catalog Number: XP-5133
Alternative Catalog Number: PRS-XP-5133-0.1
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ELISA, NeA, WB
Species Reactivity: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17).
Conjugation: Unconjugated
Alternative Names: HH20, FGF-13Fibroblast growth factor 17FGF-17
Clonality: Polyclonal
Concentration: batch dependent
NCBI: 8822
UniProt: O60258
Form: Lyophilized
Application Dilute: Centrifuge vial prior to opening.
Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/mL), a concentration of 0.02 - 0.035 µg/mL of this antibody is required. ELISA:To detect hFGF-17 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-17 (XP-5133Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot:To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.