flt3-Ligand Antibody (biotin), Biotin, Rabbit, Polyclonal

Produktübersicht

• Artikelname: flt3-Ligand Antibody (biotin), Biotin, Rabbit, Polyclonal
• Artikelnummer: PRS-XP-5138BT
• Hersteller Artikelnummer: XP-5138Bt
• Alternativnummer: PRS-XP-5138BT-0.05
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt3-Ligand.
• Konjugation: Biotin
• Alternative Synonym: FL, FLT3LFms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 ligand

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 2323
• UniProt: P49771
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/mL), a concentration of 4.0-6.0 ng/mL of this antibody is required.ELISA:Sandwich:To detect hFlt-3 Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Flt-3 Ligand (XP-5138Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.Western Blot:To detect hFlt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.