flt3-Ligand Antibody (biotin), Biotin, Rabbit, Polyclonal
Catalog Number:
PRS-XP-5138BT
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| Article Name: | flt3-Ligand Antibody (biotin), Biotin, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-XP-5138BT |
| Supplier Catalog Number: | XP-5138Bt |
| Alternative Catalog Number: | PRS-XP-5138BT-0.05 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, NeA, WB |
| Species Reactivity: | Human |
| Immunogen: | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt3-Ligand. |
| Conjugation: | Biotin |
| Alternative Names: | FL, FLT3LFms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 ligand |
| Application Dilute: | Centrifuge vial prior to opening. |
| Application Notes: | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/mL), a concentration of 4.0-6.0 ng/mL of this antibody is required.ELISA:Sandwich:To detect hFlt-3 Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Flt-3 Ligand (XP-5138Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.Western Blot:To detect hFlt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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