Midkine Antibody (biotin), Biotin, Rabbit, Polyclonal

Artikelnummer: PRS-XP-5230BT
Artikelname: Midkine Antibody (biotin), Biotin, Rabbit, Polyclonal
Artikelnummer: PRS-XP-5230BT
Hersteller Artikelnummer: XP-5230Bt
Alternativnummer: PRS-XP-5230BT-0.05
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant (human Midkine).
Konjugation: Biotin
Alternative Synonym: MK, ARAP, NEGF2, MK1, Midkine, Amphiregulin-associated protein, MK
Klonalität: Polyclonal
Konzentration: batch dependent
NCBI: 4192
UniProt: P21741
Formulierung: Lyophilized
Application Verdünnung: Centrifuge vial prior to opening.
Anwendungsbeschreibung: ELISA:Sandwich:To detect hMidkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Midkine (XP-5230) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot:To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.