Midkine Antibody (biotin), Biotin, Rabbit, Polyclonal
Catalog Number:
PRS-XP-5230BT
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| Article Name: | Midkine Antibody (biotin), Biotin, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-XP-5230BT |
| Supplier Catalog Number: | XP-5230Bt |
| Alternative Catalog Number: | PRS-XP-5230BT-0.05 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant (human Midkine). |
| Conjugation: | Biotin |
| Alternative Names: | MK, ARAP, NEGF2, MK1, Midkine, Amphiregulin-associated protein, MK |
| Application Dilute: | Centrifuge vial prior to opening. |
| Application Notes: | ELISA:Sandwich:To detect hMidkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Midkine (XP-5230) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot:To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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