MIG Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MIG Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-XP-5231
• Hersteller Artikelnummer: XP-5231
• Alternativnummer: PRS-XP-5231-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG (human MIG).
• Konjugation: Unconjugated
• Alternative Synonym: CMK, MIG, Humig, SCYB9, crg-10, CMK, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 4283
• UniProt: Q07325
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 µg/mL of this antibody is required. ELISA:To detect hMIG by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG. Sandwich:To detect hMIG by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.Western Blot:To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.