MIG Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-XP-5231
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| Article Name: | MIG Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-XP-5231 |
| Supplier Catalog Number: | XP-5231 |
| Alternative Catalog Number: | PRS-XP-5231-0.1 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, NeA, WB |
| Species Reactivity: | Human |
| Immunogen: | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG (human MIG). |
| Conjugation: | Unconjugated |
| Alternative Names: | CMK, MIG, Humig, SCYB9, crg-10, CMK, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG |
| Application Dilute: | Centrifuge vial prior to opening. |
| Application Notes: | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 µg/mL of this antibody is required. ELISA:To detect hMIG by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG. Sandwich:To detect hMIG by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.Western Blot:To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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