NT-3 Antibody, Unconjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: NT-3 Antibody, Unconjugated, Goat, Polyclonal
• Artikelnummer: PRS-XP-5249
• Hersteller Artikelnummer: XP-5249
• Alternativnummer: PRS-XP-5249-0.1
• Hersteller: ProSci
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-3 (human Neurotrophin-3).
• Konjugation: Unconjugated
• Alternative Synonym: NT3, HDNF, NGF2, NT-3, NGF-2Neurotrophin-3

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 4908
• UniProt: P20783
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hNT-3 (50.00 ng/mL), a concentration of 2.0 - 4.0 µg/mL of this antibody is required. ELISA:To detect hNT-3 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNT-3. Sandwich:To detect hNT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NT-3 (XP-5249Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3. Western Blot:To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.