NT-3 Antibody, Unconjugated, Goat, Polyclonal

Product Overview

• Article Name: NT-3 Antibody, Unconjugated, Goat, Polyclonal
• Biozol Catalog Number: PRS-XP-5249
• Supplier Catalog Number: XP-5249
• Alternative Catalog Number: PRS-XP-5249-0.1
• Manufacturer: ProSci
• Host: Goat
• Category: Antikörper
• Application: ELISA, NeA, WB
• Species Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-3 (human Neurotrophin-3).
• Conjugation: Unconjugated
• Alternative Names: NT3, HDNF, NGF2, NT-3, NGF-2Neurotrophin-3

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• NCBI: 4908
• UniProt: P20783
• Form: Lyophilized

Application Notes

• Application Dilute: Centrifuge vial prior to opening.
• Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hNT-3 (50.00 ng/mL), a concentration of 2.0 - 4.0 µg/mL of this antibody is required. ELISA:To detect hNT-3 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNT-3. Sandwich:To detect hNT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NT-3 (XP-5249Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3. Western Blot:To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.