Endothelial Growth Factor-165 Recombinant Protein has been tested by SDS-PAGE and bioactivity and is suitable as a control for polyclonal or monoclonal anti-Endothelial Growth Factor-165 in immunological assays.
Bioactivity of Human Vascular Endothelial Growth Factor-165 Animal Free Recombinant Bioactivity of Protein. Serial dilutions of Human VEGF165 AF, starting at 50 ng/mL, were added to HUVECs. After 24 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human VEGF165 AFis 1.8-2.6 ng/mL. This value is comparable to the typical expected range of 1-6 ng/mL.
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