rHuman VEGF-165 Protein

Catalog Number: ROC-009-F01-B99-0010
Article Name: rHuman VEGF-165 Protein
Biozol Catalog Number: ROC-009-F01-B99-0010
Supplier Catalog Number: 009-F01-B99-0010
Alternative Catalog Number: ROC-009-F01-B99-0010
Manufacturer: Rockland Immunochemicals
Category: Proteine/Peptide
Application: SDS-PAGE
Conjugation: Unconjugated
Alternative Names: VEGF-A, glioma-derived endothelial cell mitogen, Vascular permeability factor (VPF)
NCBI: 15692
UniProt: P15692
Buffer: 0.1% Trifluoroacetic acid
Source: Human
Form: Lyophilized
Application Notes: Endothelial Growth Factor-165 Recombinant Protein has been tested by SDS-PAGE and bioactivity and is suitable as a control for polyclonal or monoclonal anti-Endothelial Growth Factor-165 in immunological assays.
Bioactivity of Human Vascular Endothelial Growth Factor-165 Animal Free Recombinant Bioactivity of Protein. Serial dilutions of Human VEGF165 AF, starting at 50 ng/mL, were added to HUVECs. After 24 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human VEGF165 AFis 1.8-2.6 ng/mL. This value is comparable to the typical expected range of 1-6 ng/mL.