0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Quelle:
Mouse
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
ELISA: User Optimized, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Mouse IgM kappa isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). MW: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). Lane 2: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 3: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa Myeloma Protein (p/n 010-001-033). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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