Mouse IgM Kappa isotype Control, Clone: [MMK], Monoclonal

Product Overview

• Article Name: Mouse IgM Kappa isotype Control, Clone: [MMK], Monoclonal
• Biozol Catalog Number: ROC-010-001-339
• Supplier Catalog Number: 010-001-339
• Alternative Catalog Number: ROC-010-001-339
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Mouse immunoglobulin M kappa, Mouse IgM isotype control, mouse IgM kappa

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0mg/mL by UV absorbance at 280 nm
• Clone Designation: [MMK]
• Isotype: IgM
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Source: Mouse
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized, WB: User Optimized
• Application Notes: Mouse IgM kappa isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Images

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). MW: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). Lane 2: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 3: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa Myeloma Protein (p/n 010-001-033). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.