Sonic Hedgehog Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.
Bioactivity of Mouse Sonic Hedgehog Recombinant Protein. Serial dilutions of Mouse SHH, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse SHH is 0.48-0.72 ug/mL. This value is comparable to the typical expected range of human SHH is 0.8-1 ug/mL.
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