rMouse Sonic Hedgehog Protein

Catalog Number: ROC-010-001-W24-0005
Article Name: rMouse Sonic Hedgehog Protein
Biozol Catalog Number: ROC-010-001-W24-0005
Supplier Catalog Number: 010-001-W24-0005
Alternative Catalog Number: ROC-010-001-W24-0005
Manufacturer: Rockland Immunochemicals
Category: Proteine/Peptide
Conjugation: Unconjugated
Alternative Names: HHG-1
NCBI: 62226
UniProt: Q62226
Buffer: 0.01 M Sodium Phosphate, pH 7.5
Source: Mouse
Form: Lyophilized
Application Notes: Sonic Hedgehog Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.
Bioactivity of Mouse Sonic Hedgehog Recombinant Protein. Serial dilutions of Mouse SHH, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse SHH is 0.48-0.72 ug/mL. This value is comparable to the typical expected range of human SHH is 0.8-1 ug/mL.