Mouse IgG2a isotype control Biotin, Clone: [M2A], Monoclonal

Produktübersicht

• Artikelname: Mouse IgG2a isotype control Biotin, Clone: [M2A], Monoclonal
• Artikelnummer: ROC-010-0641
• Hersteller Artikelnummer: 010-0641
• Alternativnummer: ROC-010-0641
• Hersteller: Rockland Immunochemicals
• Kategorie: Antikörper
• Applikation: ELISA
• Konjugation: Biotin
• Alternative Synonym: biotin conjugated Mouse IgG2a subclass isotype control, Mouse IgG2a isotype control biotin conjugation

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [M2A]
• Isotyp: IgG2a
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Quelle: Mouse
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: 1:1000-1:5000
• Anwendungsbeschreibung: Mouse IgG2a isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Bilder

ELISA Results of Mouse IgG2a Isotype Control Biotin Conjugated. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a Isotype Control Biotin Conjugate. The working dilution is 364,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).