0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Mouse
Form:
Lyophilized
Application Dilute:
FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: 1:1000-1:5000
Application Notes:
Mouse IgG2a isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
ELISA Results of Mouse IgG2a Isotype Control Biotin Conjugated. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a Isotype Control Biotin Conjugate. The working dilution is 364,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).
* VAT and and shipping costs not included. Errors and price changes excepted
