Rat IgG1 isotype control Phycoerythrin, Clone: [RG1], RPE, Monoclonal

Artikelnummer: ROC-012-008-040
Artikelname: Rat IgG1 isotype control Phycoerythrin, Clone: [RG1], RPE, Monoclonal
Artikelnummer: ROC-012-008-040
Hersteller Artikelnummer: 012-008-040
Alternativnummer: ROC-012-008-040
Hersteller: Rockland Immunochemicals
Kategorie: Antikörper
Applikation: DOT
Spezies Reaktivität: Rat
Konjugation: RPE
Alternative Synonym: Rat Isotype Control, Rat IgG1 PE isotype, RAT IgG1 subclass isotype control
Klonalität: Monoclonal
Konzentration: 0.5 mg/mL by UV absorbance = 82.0 at 565 nm
Klon-Bezeichnung: [RG1]
Isotyp: IgG1
Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Quelle: Rat
Formulierung: Lyophilized
Application Verdünnung: ELISA: 1:2000 - 1:20,000, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized
Anwendungsbeschreibung: RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimiz
Dot Blot results of Rat IgG1 isotype control Phycoerythrin Conjugated. Antigen: Rat IgG1 isotype control RPE Conjugated. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Imaging System ChemiDoc, Filter used: Rhodamine.