Rat IgG1 isotype control Phycoerythrin, Clone: [RG1], RPE, Monoclonal
Biozol Catalog Number:
ROC-012-008-040
Supplier Catalog Number:
012-008-040
Alternative Catalog Number:
ROC-012-008-040
Manufacturer:
Rockland Immunochemicals
Category:
Antikörper
Application:
DOT
Species Reactivity:
Rat
Conjugation:
RPE
Alternative Names:
Rat Isotype Control, Rat IgG1 PE isotype, RAT IgG1 subclass isotype control
Clonality:
Monoclonal
Concentration:
0.5 mg/mL by UV absorbance = 82.0 at 565 nm
Clone Designation:
[RG1]
Isotype:
IgG1
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Source:
Rat
Form:
Lyophilized
Application Dilute:
ELISA: 1:2000 - 1:20,000, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized
Application Notes:
RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimiz
Dot Blot results of Rat IgG1 isotype control Phycoerythrin Conjugated. Antigen: Rat IgG1 isotype control RPE Conjugated. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Imaging System ChemiDoc, Filter used: Rhodamine.
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