Choline Oxidase Antibody, Goat, Polyclonal

Artikelnummer: ROC-100-101-094
Artikelname: Choline Oxidase Antibody, Goat, Polyclonal
Artikelnummer: ROC-100-101-094
Hersteller Artikelnummer: 100-101-094
Alternativnummer: ROC-100-101-094
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Bacteria
Immunogen: Choline Oxidase [Alcaligenes species]
Konjugation: Unconjugated
Alternative Synonym: Choline oxidase, goat anti-Choline Oxidase Antibody
Klonalität: Polyclonal
Konzentration: 90 mg/mL by Refractometry
NCBI: 116367
UniProt: P16101
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Alcaligenes
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Anwendungsbeschreibung: Goat anti-Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code
Rockland Goat anti Choline Oxidase antibody was used to detect Choline Oxidase. Samples of ~1 and 0.25 µg of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline Oxidase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti-Choline Oxidase (p/n 200-101-094, lot 5911) at 1:5K in (p/n MB-070) ON at 4C. Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti-Goat (p/n 605-743-125) at 1:10K for 1.5 hr RT in (p/n MB-070) and imaged on the BioRad VersaDoc imaging system.