Choline Oxidase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Choline Oxidase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-100-101-094
• Supplier Catalog Number: 100-101-094
• Alternative Catalog Number: ROC-100-101-094
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: Choline Oxidase [Alcaligenes species]
• Conjugation: Unconjugated
• Alternative Names: Choline oxidase, goat anti-Choline Oxidase Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 116367
• UniProt: P16101
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Alcaligenes
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Goat anti-Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code

Images

Rockland Goat anti Choline Oxidase antibody was used to detect Choline Oxidase. Samples of ~1 and 0.25 µg of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline Oxidase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti-Choline Oxidase (p/n 200-101-094, lot 5911) at 1:5K in (p/n MB-070) ON at 4C. Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti-Goat (p/n 605-743-125) at 1:10K for 1.5 hr RT in (p/n MB-070) and imaged on the BioRad VersaDoc imaging system.