Adenosine Deaminase Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Adenosine Deaminase Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-100-401-140
• Hersteller Artikelnummer: 100-401-140
• Alternativnummer: ROC-100-401-140
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Bovine, Human, Mouse
• Immunogen: Adenosine Deaminase [Calf Spleen]
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 90 mg/mL by Refractometry
• NCBI: 280712
• UniProt: P56658
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Bovine
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,000
• Anwendungsbeschreibung: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Bilder

Rockland Anti-adenosine deaminase biotin conjugated (200-406-140) was used to detect adenosine deaminase. Lane 1: mouse pancreas lysate detect purified. Lane 2: Jurkat and Raji lysate (1:1 mixture) detect purified. Lane 3: Jurkat and Raji lysate (1:1 mixture) detect endogenous. Lane 4: Mouse Pancreas and Liver lysate (1:1 mixture). Load: 30µL. Blocked with 3% Fish Gel (left) or 3% BSA (right). Primary Antibody: Anti-Adenosine Deaminase biotin conjugated (p/n 200-406-140) lot 5628 (1:5K in TBS, Overnight at 4C). Secondary Antibody: Dylight 488 conjugated Streptavidin (p/n S000-41 lot 20833 1:5K in MB-070 for 30 minutes). Blot was imaged with the Biorad VersaDoc imaging system.