Adenosine Deaminase Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Adenosine Deaminase Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-140
• Supplier Catalog Number: 100-401-140
• Alternative Catalog Number: ROC-100-401-140
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Bovine, Human, Mouse
• Immunogen: Adenosine Deaminase [Calf Spleen]
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Product Properties

• Clonality: Polyclonal
• Concentration: 90 mg/mL by Refractometry
• NCBI: 280712
• UniProt: P56658
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,000
• Application Notes: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Images

Rockland Anti-adenosine deaminase biotin conjugated (200-406-140) was used to detect adenosine deaminase. Lane 1: mouse pancreas lysate detect purified. Lane 2: Jurkat and Raji lysate (1:1 mixture) detect purified. Lane 3: Jurkat and Raji lysate (1:1 mixture) detect endogenous. Lane 4: Mouse Pancreas and Liver lysate (1:1 mixture). Load: 30µL. Blocked with 3% Fish Gel (left) or 3% BSA (right). Primary Antibody: Anti-Adenosine Deaminase biotin conjugated (p/n 200-406-140) lot 5628 (1:5K in TBS, Overnight at 4C). Secondary Antibody: Dylight 488 conjugated Streptavidin (p/n S000-41 lot 20833 1:5K in MB-070 for 30 minutes). Blot was imaged with the Biorad VersaDoc imaging system.