ERK2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ERK2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-100-401-218
• Hersteller Artikelnummer: 100-401-218
• Alternativnummer: ROC-100-401-218
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Erk2 Antibody, MAP kinase 1, MAP kinase 2, p42-MAPK

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 85 mg/mL by Refractometry
• NCBI: 5594
• UniProt: P28482
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IP: 5.0 µg/mg sample, WB: 1:1,000-1:,2000
• Anwendungsbeschreibung: This antiserum was tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in t

Bilder

Western blot using Rocklands Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates. Lane 1: HeLa (p/n W09-000-364), Lane 2: A431 (p/n W09-000-361), and Lane 3: NIH3T3 (p/n W10-000-358). Primary antibody: Anti-p42 at 1:1,000. Secondary antibody: HRP Goat-a-Rabbit 1:4,000 dilution with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results.