Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
This antiserum was tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in t
Western blot using Rocklands Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates. Lane 1: HeLa (p/n W09-000-364), Lane 2: A431 (p/n W09-000-361), and Lane 3: NIH3T3 (p/n W10-000-358). Primary antibody: Anti-p42 at 1:1,000. Secondary antibody: HRP Goat-a-Rabbit 1:4,000 dilution with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results.
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