DRIP130 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-401-239
Artikelname: DRIP130 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-401-239
Hersteller Artikelnummer: 100-401-239
Alternativnummer: ROC-100-401-239
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human, Mouse
Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) crosslinked to KLH.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-DRIP130 antibody, Mediator of RNA polymerase II transcription subunit 23, MED23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2
Klonalität: Polyclonal
Konzentration: 85 mg/mL by Refractometry
NCBI: 9439
UniProt: Q9ULK4
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:25,000, IP: 1:100 - 1:200, WB: 1:1,000
Anwendungsbeschreibung: Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for ap
Anti-DRIP-130 is shown to immunoprecipitate 35S-labeled in vitro translated human DRIP130 (lane 1). A similar precipitation was performed for pre-immune serum (lane 2). Immunoprecipitation occurs using a 1:100 to 1:200 dilution of the antiserum. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.