DRIP130 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: DRIP130 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-239
• Supplier Catalog Number: 100-401-239
• Alternative Catalog Number: ROC-100-401-239
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Mouse
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) crosslinked to KLH.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-DRIP130 antibody, Mediator of RNA polymerase II transcription subunit 23, MED23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2

Product Properties

• Clonality: Polyclonal
• Concentration: 85 mg/mL by Refractometry
• NCBI: 9439
• UniProt: Q9ULK4
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:25,000, IP: 1:100 - 1:200, WB: 1:1,000
• Application Notes: Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for ap

Images

Anti-DRIP-130 is shown to immunoprecipitate 35S-labeled in vitro translated human DRIP130 (lane 1). A similar precipitation was performed for pre-immune serum (lane 2). Immunoprecipitation occurs using a 1:100 to 1:200 dilution of the antiserum. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.