Anti-ChAT antibody has been tested in western blot and IHC and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western B
Western Blot of Rabbit Anti-ChAT antibody. Lane 1: rat brain. Lane 2: none. Load: 10 µg per lane. Primary antibody: Choline Acetyltransferase antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~68/70k,~68/70k for choline acetyltransferase protein. Other band(s): ChAT splice variants and isoforms.
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