ChAT (Choline Acetyltransferase) Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ChAT (Choline Acetyltransferase) Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-D16
• Supplier Catalog Number: 100-401-D16
• Alternative Catalog Number: ROC-100-401-D16
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Mouse, Primate, Rat
• Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta
• Conjugation: Unconjugated
• Alternative Names: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 1103
• UniProt: P28329
• Buffer: None
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:100, WB: 1:1000
• Application Notes: Anti-ChAT antibody has been tested in western blot and IHC and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western B

Images

Western Blot of Rabbit Anti-ChAT antibody. Lane 1: rat brain. Lane 2: none. Load: 10 µg per lane. Primary antibody: Choline Acetyltransferase antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~68/70k,~68/70k for choline acetyltransferase protein. Other band(s): ChAT splice variants and isoforms.