Fractin Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-401-D40
Artikelname: Fractin Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-401-D40
Hersteller Artikelnummer: 100-401-D40
Alternativnummer: ROC-100-401-D40
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Rat
Immunogen: Anti-Fractin Antibody was produced in rabbit by repeated immunizations with Fractin- Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Konjugation: Unconjugated
Alternative Synonym: Fragment of actin
Klonalität: Polyclonal
Konzentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 60
UniProt: P60709
Puffer: None
Formulierung: Liquid
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: IHC: 1:100, WB: 1:1000
Anwendungsbeschreibung: Anti-Fractin (Rabbit) Antibody has been tested by western blot, ICC, and IHC. Excepted reactivity to the 32 kDa fractin protein in Western blots. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-Fractin (Rabbit) Antibody. Lane 1: colchicine treated Sy5y cell lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Fractin antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 32k , ~ 32k for cleaved actin fragment (fractin). Other band(s): none.