Fractin Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Fractin Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-D40
• Supplier Catalog Number: 100-401-D40
• Alternative Catalog Number: ROC-100-401-D40
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Rat
• Immunogen: Anti-Fractin Antibody was produced in rabbit by repeated immunizations with Fractin- Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Fragment of actin

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 60
• UniProt: P60709
• Buffer: None
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:100, WB: 1:1000
• Application Notes: Anti-Fractin (Rabbit) Antibody has been tested by western blot, ICC, and IHC. Excepted reactivity to the 32 kDa fractin protein in Western blots. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Rabbit Anti-Fractin (Rabbit) Antibody. Lane 1: colchicine treated Sy5y cell lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Fractin antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 32k , ~ 32k for cleaved actin fragment (fractin). Other band(s): none.