Anti-MeCP2 pS80 Antibody was produced in rabbit by repeated immunizations with MeCP Ser80 - Synthetic peptide corresponding to amino acid residues surrounding Ser 80 conjugated to KLH.
Konjugation:
Unconjugated
Alternative Synonym:
Methyl-CpG-binding protein 2, MeCp-2 protein
Klonalität:
Polyclonal
Konzentration:
Titrated value sufficient to run approximately 10 mini blots.
Anti-MeCP2 pS80 (Rabbit) has been tested in Western Blotting and IHC. Expect ~75kDa in WB. Anti-MeCP2 pS80 antibodies have been used successfully for IHS on mouse brain sections. Specific conditions for reactivity should be optimized by the end user. Res
Western Blot of Rabbit Anti-MeCP2 pS80 Antibody. Lane 1: neonatal rat brain. Lane 2: none. Load: 10 µg per lane. Primary antibody: MeCP2 pS80 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 75k , ~ 75k for MeCP2 protein phosphorylated at Ser80. Other band(s): none.
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