MeCP2 Phospho S80 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: MeCP2 Phospho S80 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-D71
• Supplier Catalog Number: 100-401-D71
• Alternative Catalog Number: ROC-100-401-D71
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-MeCP2 pS80 Antibody was produced in rabbit by repeated immunizations with MeCP Ser80 - Synthetic peptide corresponding to amino acid residues surrounding Ser 80 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Methyl-CpG-binding protein 2, MeCp-2 protein

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 4204
• UniProt: P51608
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:100, WB: 1:1000
• Application Notes: Anti-MeCP2 pS80 (Rabbit) has been tested in Western Blotting and IHC. Expect ~75kDa in WB. Anti-MeCP2 pS80 antibodies have been used successfully for IHS on mouse brain sections. Specific conditions for reactivity should be optimized by the end user. Res

Images

Western Blot of Rabbit Anti-MeCP2 pS80 Antibody. Lane 1: neonatal rat brain. Lane 2: none. Load: 10 µg per lane. Primary antibody: MeCP2 pS80 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 75k , ~ 75k for MeCP2 protein phosphorylated at Ser80. Other band(s): none.